Gyuna Kim

Conformationally dynamic proteins drive key cellular processes, behaviors, and functional outputs. Through the understanding of key residue interactions of a common protein motif, we can both design conformationally dynamic proteins and gain a generalized understanding of the sequence-structure-function relationship.

Conformationally dynamic proteins drive key cellular processes, behaviors, and functional outputs. Through the understanding of key residue interactions of a common protein motif, we can both design conformationally dynamic proteins and gain a generalized understanding of the sequence-structure-function relationship. Switch proteins will be designed using a rational design approach informed by well-understood coiled-coil sequence-structure relationships. Novel application of high-throughput screening approaches will be used to screen for candidate switches at library scale and extract information about equilibria between switch states. Together these data will allow us to map the space of engineerable helix sliding switches and lay the groundwork for precisely tuning protein switches.